The human prostate cancer antigen 3 (PCA3) is a long non-coding RNA (lncRNA) commonly used as a diagnostic marker for prostate cancer (PCa). Herein we investigated the cellular function of PCA3 in PCa and its potential mechanism.
PCA3 was overexpressed in a PC3 cell line (PC3(PCA+)) and cell proliferation, migration, invasion and apoptosis were compared to those of control cells (PC3(NC)). Differentially expressed proteins were identified by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry.
Overexpression of PCA3 significantly increased cell proliferation rate, migration and invasion, while inhibited apoptosis in PC3 cells. Three proteins were found down-regulated and 7 proteins up-regulated in PC3(PCA+) cells compared to PC3(NC) cells, including GRP78. Higher GRP78 was also found in PCa clinical specimens.
This study confirmed that in PCa, PCA3 plays a pro-cancer role through promoting cell proliferation, migration and invasion while inhibiting cell apoptosis. This process might involve the up-regulation of GRP78.
Anticancer research. 2017 Aug [Epub]
Chunhua Lin, Jiahui Wang, Yanwei Wang, Peng Zhu, Xin Liu, Ning Li, Juan Liu, Luxin Yu, Wenting Wang
Department of Urological Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China., Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China., Department of Urological Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China ., Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China .