eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients

The purpose of the present study was to investigate whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in prostate cancer (PCa).

We examined three eNOS gene polymorphisms (T-786C promoter region, G894T, and Intron 4 VNTR 4a/b) at extracted DNAs from 50 formalin-fixed paraffin-embedded tissues of PCa patients. For the controls, blood samples obtained from 50 healthy men were studied. Genotyping of molecular variants was performed by PCR-RFLP technique.

We found that the TC genotype of the T-786C polymorphism was associated with PCa risk (OR: 3.325, CI: 1.350-8.188, P = 0.008). The eNOS G894T polymorphism was also associated with PCa. The frequency of the 894T allele was significantly higher in PCa patients. No association was identified between intron 4 VNTR polymorphism and PCa.

We found significant differences in genotypic and allelic frequencies between PCa patients and controls for eNOS T-786C and G894T polymorphisms. The presence of the T-786C genotype and 894T allele in carriers increased the risk of PCa. No association was found between intron 4 VNTR polymorphism and PCa patients.

Turkish journal of medical sciences. 2016 Apr 19*** epublish ***

Fikriye Polat, Nesrin Turaçlar, Meral Yilmaz, Günsel Bingöl, Hasibe Cingilli Vural

Department of Primary Education, Faculty of Education, Elementary Sciences Education, Kocaeli University, Campus of Umuttepe, Kocaeli, Turkey., Vocational School of Health Services, Selçuk University, Konya, Turkey., Department of Research Centre, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey., Biomedical Engineering, Faculty of Engineering and Natural Sciences, Yıldırım Beyazıt University, Ankara, Turkey., Department of Biology, Molecular Biology, Selçuk University, Selçuklu, Konya, Turkey.