Abnormal microRNA (miR) expressions were implicated in prostate cancer progression. We identified a novel miR-495, which was downregulated in prostate cancer, but not normal prostate cell lines. MiR-495 directly targeted the 3'-UTR of Akt and mTOR mRNAs.
Expression of miR-495 in prostate cancer cells significantly downregulated Akt and mTOR, which further inhibited cancer cell proliferation, migration, and invasion in vitro. Function of miR-495 in vivo was examined in mouse xenograft model and was found to significantly inhibit the growth of tumors, mediated by repressing Akt and mTOR. Our report supported miR-495 as a novel tumor suppressor microRNA in prostate cancer.
Cancer investigation. 2016 Mar 31 [Epub ahead of print]
Jian-Zhang Li, Zhen-Long Wang, Wan-Hai Xu, Qing Li, Lin Gao, Zi-Ming Wang
a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China., b Department of Urology , The Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China., a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China., a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China., a Department of Urology , The Fourth Affiliated Hospital of Harbin Medical University , Harbin , China., b Department of Urology , The Second Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.