Metastatic dissemination is a feature of most cancers including prostate cancer (PCa), and is the main cause of treatment failure and mortality. The aim of the study is to explore the mechanisms of PCa metastasis and to search for potential prognostic markers using proteomics.
Two-dimensional fluorescent differential gel electrophoresis (2D-DIGE) was used to quantify proteins in normal prostate epithelial cells, bone metastasis-derived PC-3 cells, and visceral metastasis-derived PC-3M cells. Metastatic potential was confirmed by flow cytometry, electron microscopy, proliferating cell nuclear antigen assay, and wound healing assay. Differential protein expression was compared between PCa cells with different metastatic potentials (LNcap, DU145, PC-3 and PC-3M) and normal prostate epithelial cells (RWPE-1). Selected candidate proteins in human prostate tissues were analyzed using GOA, UniProt and GeneCards analyses. Eighty-six proteins were differentially expressed between cell lines (>1. 5-fold, P<0. 05). Among them, twelve proteins were identified by MALDI-TOF-MS. One protein was upregulated in normal prostate epithelial cells, nine proteins were upregulated in PC-3, and two proteins were upregulated in PC-3M. Proteins were divided into five groups according to their functions. The SETDB1 protein was closely associated with the prognosis of PCa. Bioinformatics suggested that SETDB1 might promote PCa bone metastasis through the WNT pathway. In conclusion, SETDB1 might be associated with the development of bone metastases from PCa. Further study is necessary to assess its exact role in PCa.
International journal of oncology. 2016 Feb 04 [Epub ahead of print]
Qun Li, Yilei Li, Yanying Wang, Zheng Cui, Lulu Gong, Zhigang Qu, Yanping Zhong, Jun Zhou, Ying Zhou, Yong Gao, Yulin Li
Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P. R. China. , The Key Laboratory of Pathobiology, Ministry of Education, Bethune Medical School, Jilin University, Changchun, Jilin 130021, P. R. China. , Department of Endoscopy in Special Clinic Room, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P. R. China. , Department of Ultrasound, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P. R. China. , The Hepatobiliary Pancreatic Surgery, Second Hospital of Jilin University, Changchun, Jilin 130041, P. R. China. , The Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P. R. China. , Department of Pathology, The First Hospital of Jilin University, Changchun, Jilin 130021, P. R. China. , Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P. R. China. , Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P. R. China. , Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P. R. China. , The Key Laboratory of Pathobiology, Ministry of Education, Bethune Medical School, Jilin University, Changchun, Jilin 130021, P. R. China.