B7-H3 over expression in prostate cancer promotes tumor cell progression - Abstract

Department of Urology, Suzhou, People's Republic of China.

 

We compared B7-H3 expression in benign prostatic hyperplasia and prostate cancer tissue specimens, and determined the effects of low B7-H3 expression on the PC-3 human prostate cancer cell line using RNA interference.

B7-H3 expression in prostate specimens was determined by enzyme-linked immunosorbent assay. A PC-3 cell line with low B7-H3 expression was established by RNA interference to investigate the effect of B7-H3 on cell proliferation, adhesion, migration and invasion in vitro.

B7-H3 in tissue samples was significantly higher in the prostate cancer group than in the benign prostatic hyperplasia group (mean ± SEM 174.73 ± 56.80 vs 82.69 ± 46.19 ng/gm, p < 0.001). B7-H3 expression down-regulated by small interfering RNA decreased cell adhesion to PC-3 fibronectin more than 30%, and migration and Matrigel™ invasion up to 50%. No apparent impact was observed on cell proliferation.

B7-H3 is aberrantly expressed in prostate cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating PC-3 cell progression.

Written by:
Yuan H, Wei X, Zhang G, Li C, Zhang X, Hou J.   Are you the author?

Reference: J Urol. 2011 Jul 22. Epub ahead of print.
doi: 10.1016/j.juro.2011.04.103

PubMed Abstract
PMID: 21784485

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