miR-218-5p is an extensively studied microRNA (miRNA) in prostate cancer (PCa). However, the clinical significance and biological role of miR-218-5p in bone metastasis of PCa remain unclear.
miR-218-5p expression was evaluated in 38 bone metastatic and 115 non-bone metastatic PCa tissues and serum samples. Clinical correlation of miR-218-5p expression with clinicopathological characteristics was analyzed. The biological roles of miR-218-5p in bone metastasis of PCa were investigated in vitro by invasion and migration assays. Bioinformatics analysis, real-time polymerase chain reaction, western blot, and luciferase reporter assay were applied to discern and examine the relationship between miR-218-5p and its potential targets.
miR-218-5p expression was reduced in bone metastatic PCa tissue and serum samples, which positively correlated with poor clinicopathological characteristics and bone metastasis-free survival in PCa patients. Upregulating miR-218-5p repressed PCa cell invasion and migration. Furthermore, miR-218-5p inhibited NF-κB signaling via simultaneously targeting TRAF1, TRAF2, and TRAF5, which suppressed the invasion and migration abilities of PCa cells. ROC curve analysis of miR-218-5p in the serum of PCa patients exhibited an area under the curve of 0.86 (95% confidence interval 0.80-0.92, p < 0.001).
Our findings indicate that miR-218-5p might represent a novel serum biomarker for bone metastasis of PCa.
Oncology research and treatment. 2019 Mar 14 [Epub ahead of print]
Peng Peng, Tao Chen, Qing Wang, Yixi Zhang, Fangfang Zheng, Shuai Huang, Yubo Tang, Chunxiao Yang, Wenqing Ding, Dong Ren, Zongwen Huang, Yuanqing Guo
Department of Orthopedic Surgery, Zhuhai Second People's Hospital, Zhuhai, China., Department of Orthopedic Surgery, The Fifth Hospital Affiliated of Sun Yat-sen University, Zhuhai, China., Department of Orthopedic Surgery, The Affiliated Hospital of Zunyi Medical College, Zunyi, China., Department of Ophthalmology, Zhuhai Second People's Hospital, Zhuhai, China., Department of Pediatrics, The Fifth Hospital Affiliated of Sun Yat-sen University, Zhuhai, China., Department of Orthopedic Surgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Department of Pharmacy, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Department of Clinical Cytogenetics, Suzhou Precision Medicine Scientific Ltd., Suzhou, China., Department of Orthopedic Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Department of Orthopedic Surgery, The Fifth Hospital Affiliated of Sun Yat-sen University, Zhuhai, .