Objective To investigate the expression of E2F transcription factor 3 (E2F3) and the infiltration of CD8+T cells in bladder cancer tissues and their correlations with the malignant biological behaviors of bladder cancer. Methods The expression level of E2F3 and the infiltration characteristics of CD8+T cells in 110 cases of bladder cancer tissues with different histological stage and grade as well as in the corresponding normal bladder tissues were detected by immunohistochemistry. Western blot analysis was used to detect E2F3 and CD8 protein levels in 82 cases of bladder cancer tissues and the corresponding normal bladder tissues, and then the correlation between E2F3 expression and CD8+T cell infiltration was analyzed statistically by linear correlation analysis. Results The expression of E2F3 was higher in the bladder cancer tissues than in the normal bladder tissues. The expression level of E2F3 increased gradually with the increase of tumor histological stage and grade, while the number of tumor-infiltrating CD8+T cells decreased gradually in the corresponding tumors, and the number of tumor-infiltrating CD8+T cells in E2F3 high expression group was significantly lower than that in E2F3 low expression group. Both the rate of E2F3 positive expression and the decrease in the number of tumor-infiltrating CD8+T cells were closely related to tumor clinical invasive indicators, such as tumor histological stage, grade, tumor size, lymph node metastasis or distant metastasis. Linear correlation analysis indicated that the number of tumor-infiltrating CD8+T cells was negatively correlated with the expression of E2F3. Survival analysis revealed that the prognosis of patients with high E2F3 expression was worse after operation within 5 years. Conclusion The expression level of E2F3 in bladder cancer is closely related to tumor invasiveness, and is negatively correlated with the number of tumor-infiltrating CD8+T cells.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2018 Jan [Epub]
Chengyuan Wang, Zhibin Xu, Wei Li, Yong Jiao, Jangping Wang, Bo Zhang
Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038; Department of Surgery, Baoji Municipal Central Hospital, Baoji 721008, China., Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China., Department of Histology and Embryology, College of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China., Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. *Corresponding author, E-mail: .