Bladder cancer (BC) has a high recurrence rate and may progress to being a muscle-invasive lesion, that is potentially associated with a poor prognosis. We identified tumor-associated proteins that were recognized by autoantibodies in sera from patients with high-grade non-muscle-invasive bladder cancer (HG-NMIBC) by proteomic analysis.
The serum levels of these autoantibodies against identified proteins were validated by dot blot analysis with sera from 95 patients with BC and 35 healthy controls. The expression of identified proteins was immunohistochemically analyzed in 115 BC tissues.
Autoantibody against protein phosphatase 1, catalytic subunit, alpha isoform (PPP1CA) protein was detected in pretreated sera from patients with HG-NMIBC who showed progression. The serum IgG level of anti-PPP1CA autoantibody was significantly correlated with pathological stage, grade, lymphovascular invasion, and prognosis. The immunoreactions for PPP1CA protein in BC was significantly correlated with pathological stage, grade, and lymphovascular invasion.
PPP1CA is a candidate sero-diagnostic and prognostic marker for patients with BC.
Anticancer research. 2017 Dec [Epub]
Sho Minami, Kazumasa Matsumoto, Ryo Nagashio, Daisuke Hagiuda, Eriko Fukuda, Naoki Goshima, Manabu Hattori, Benio Tsuchiya, Kazuo Hachimura, Shi-Xu Jiang, Makoto Saegusa, Masatsugu Iwamura, Yuichi Sato
Department of Applied Tumor Pathology, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Japan., Department of Urology, School of Medicine, Kitasato University, Sagamihara, Japan ., Functional Proteomics Team, Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan., Department of Molecular Diagnosis, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan., Department of Pathology, School of Medicine, Kitasato University, Sagamihara, Japan., Department of Urology, School of Medicine, Kitasato University, Sagamihara, Japan.