We aimed to investigate the role of miR-301b in the modulation of the proliferation, migration and invasion in bladder cancer (BLCA) cells. The expression of miR-301b and EGR1 (early growth response gene 1) mRNA were analyzed by qRT-PCR (quantitative real-time polymerase chain reaction). Dual-luciferase reporter gene system was used to identify the target relationship between miR-301b and EGR1. Cell proliferation, cell cycle and apoptosis were analyzed by MTT assay, colony-forming assay and flow cytometry, respectively. Cell motility and invasion were assessed by wound healing and transwell assays. The expression of proteins involved in epithelial-to-mesenchymal transition (EMT) and EGR1 were determined by western blot. Our results showed that miR-301b was up-regulated while EGR1 was down-expressed in BLCA tissues compared with adjacent normal tissues. The proliferation, migration and invasiveness of T24 cells (a kind of human BLCA cells) were suppressed by decreasing miR-301b expression or increasing EGR1 expression. In addition, miR-301b promoted EMT signaling by influencing the expression of related proteins. In conclusion, miR-301b could promote the proliferation, migration and aggressiveness of human BLCA cells by inhibiting the expression of EGR1.
Biochemistry and cell biology = Biochimie et biologie cellulaire. 2017 May 18 [Epub ahead of print]
Lei Yan, Yan Wang, Jun Liang, Zhixin Liu, Xiaodong Sun, Kerui Cai
Mudanjiang Medical University, Mudanjiang, China ; ., Mudanjiang Medical University, Mudanjiang, China ; ., Mudanjiang Medical University, Mudanjiang, China ; ., Mudanjiang Medical University, Mudanjiang, China ; ., Mudanjiang Medical University, Mudanjiang, China ; ., Mudanjiang Medical University, Mudanjiang, China ; .