Personalized Neoantigen Vaccines and immunotherapy for Urothelial Cancer - Expert Commentary

Most patients with urothelial cancer (UC) do not benefit from single agent immune checkpoint inhibitors (ICIs). A recent phase 1 trial by Saxena et al. evaluated the combination of atezolizumab (anti-PD-L1) with PGV001, a novel personalized neoantigen vaccine, in patients with urothelial cancer to stimulate antitumor immunity.

The primary endpoints were feasibility and safety. A vaccine was successfully prepared (median 20.3 weeks) for 10 of 12 enrolled participants, with a median of 103 neoantigens identified per patient. All participants initiating treatment completed the priming cycle. Treatment-related adverse events were predominantly grade 1, including injection site reactions (40%), fatigue (40%), and fever/chills/rigors (50%). One patient experienced immune-related hepatitis, which resolved with corticosteroids. At a median follow-up of 39 months, three of four patients treated in the adjuvant setting remained free of recurrence. In the metastatic setting, two of five patients with measurable disease achieved objective responses (40%, 95% CI: 5.3–85.3%), including one with complete radiographic resolution of liver metastases. The median progression-free survival and overall survival in the metastatic cohort were 3.5 months (95% CI: 1.5 months–not reached) and 19.1 months (95% CI: 14.1 months–not reached), respectively.

All evaluable participants demonstrated on-treatment emergence of neoantigen-specific T cell responses, with 55% (95% CI: 44.1–65.9%) of selected peptides eliciting responses. These responsive peptides induced a median 10.5-fold increase in IFNγ production from baseline. Both CD8+ and CD4+ T cell responses were observed in all evaluable patients. Notably, patients with no evidence of disease at follow-up demonstrated the largest increases in neoantigen-specific T cell activity.

This important phase I trial demonstrated that combining personalized neoantigen vaccination with atezolizumab was feasible and safe in UC patients, successfully meeting its endpoints. The treatment induced robust neoantigen-specific T cell immunity and showed promising clinical outcomes, warranting further investigation in larger clinical trials.

Written by: Bishoy M. Faltas, MD, Chief Research Officer, Englander Institute for Precision Medicine, Gellert Family - John P. Leonard, MD, Research Scholar, Associate Professor of Medicine, Cell and Developmental Biology, Weill Cornell Medicine, New York- Presbyterian Hospital, NY

  1. References: Saxena M, Anker JF, Kodysh J, O'Donnell T, Kaminska AM, Meseck M, Hapanowicz O, Niglio SA, Salazar AM, Shah HR, Kinoshita Y, Brody R, Rubinsteyn A, Sebra RP, Bhardwaj N, Galsky MD. Atezolizumab plus personalized neoantigen vaccination in urothelial cancer: a phase 1 trial. Nat Cancer. 2025 May 9. doi: 10.1038/s43018-025-00966-7.
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