Urinary stone disease is among the most common medical conditions. Standard evaluation of urinary stone disease involves a metabolic workup of stone formers based on measurement of minerals and solutes excreted in 24-hour urine samples. Nevertheless, 24-hour urine testing is slow, expensive, and inconvenient for patients, which has hindered widespread adoption in clinical practice. Here, we demonstrate SLIPS-LAB (Slippery Liquid-Infused Porous Surface Laboratory), a droplet-based bioanalysis system, for rapid measurement of urinary stone-associated analytes. The ultra-repellent and antifouling properties of SLIPS, which is a biologically inspired surface technology, allow autonomous liquid handling and manipulation of physiological samples without complicated sample preparation procedures and supporting equipment. We pilot a study that examines key urinary analytes in clinical samples from patients with urinary stone. The simplicity and speed of SLIPS-LAB hold the potential to provide actionable diagnostic information for patients with urinary stone disease and rapid feedback for responses to dietary and pharmacologic treatments.
Science advances. 2020 May 22*** epublish ***
Hui Li, Eugene Shkolyar, Jing Wang, Simon Conti, Alan C Pao, Joseph C Liao, Tak-Sing Wong, Pak Kin Wong
Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, USA., Department of Urology, Stanford University School of Medicine, Stanford, CA, USA., Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, USA., Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA., Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, USA. .