Familial members of urolithiasis have high risk for stone development. We observed the low sulfated glycosaminoglycan (GAG) excretion in urolithiasis patients and their descendants. In this study, we investigated urinary excretion of sulfated GAG, chondroitin sulfate (CS), heparan sulfate (HS) and hyaluronic acid (HA) in urolithiasis and their children, and explored the effect of CS and HA supplement in urolithic hyperoxaluric rats. The 24-hour urines were collected from urolithiasis patients (28) and their children (40), as well as healthy controls (45) and their children (33) to measure urinary sulfated GAG, CS, HS and HA excretion rate. Our result showed that urinary sulfated GAG and CS were diminished in both urolithiasis patients and their children, while decreased HS and increased HA were observed only in urolithiasis patients. Percentage of HS per sulfated GAG increased in both urolithiasis patients and their children. In hyperoxaluric rats induced by ethylene glycol and vitamin D, we found that CS supplement could prevent stone formation, while HA supplement had no effect on stone formation. Our study revealed that decreased urinary GAG and CS excretion are common in familial members of urolithiasis patients, and CS supplement might be beneficial in calcium oxalate urolithiasis prophylaxis for hyperoxaluric patients.
PloS one. 2019 Mar 07*** epublish ***
Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Monpicha Srisa-Art, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Piyaratana Tosukhowong
STAR Unit of Renal Biochemistry and Stone Disease, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand., Division of Urology, Department of Surgery, Sunpasitthiprasong Hospital, Ubon Ratchathani Province, Thailand., Division of Physiology, School of Medical Science, University of Phayao, Phayao, Thailand., Renal Immunology and Transplant Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.