Comprehensive characterization of the urinary and urothelium-bound microbiomes in bladder cancer (BCa) and healthy state is essential to understand how these local microbiomes may play a role in BCa tumorigenesis and response to therapy, as well as to explain sex-based differences in BCa pathobiology.
Performing 16 s rDNA microbiome analysis on 166 samples (urine and paired bladder tissues) from therapy-naïve BCa patients undergoing radical cystectomy and healthy controls, we defined (1) sex-specific microbiome differences in the urine and bladder tissue, and (2) representativeness of the tissue microenvironment by the voided urinary microbiome. The genus Klebsiella was more common in the urine of female BCa patients versus healthy controls, while no clinically relevant bacteria were found differently enriched in men. In tissues, the genus Burkholderia was more abundant in the neoplastic versus the non-neoplastic tissue in both sexes, suggesting a potential role in BCa pathobiology. Lastly, we found that the urinary microbiome shares >80% of the bacterial families present in the paired bladder tissue, making the urinary microbiome a fair proxy of the tissue bacterial environment. PATIENT SUMMARY: We identified specific bacteria present in the urine and tissues of male and female bladder cancer patients. These novel data represent a first step toward understanding the influence of the bladder microbiome on the development of bladder cancer and on the response to intravesical and systemic therapies.
European urology oncology. 2020 Apr 25 [Epub ahead of print]
Filippo Pederzoli, Roberto Ferrarese, Virginia Amato, Irene Locatelli, Elisa Alchera, Roberta Lucianò, Manuela Nebuloni, Alberto Briganti, Andrea Gallina, Renzo Colombo, Andrea Necchi, Massimo Clementi, Francesco Montorsi, Nicasio Mancini, Andrea Salonia, Massimo Alfano
Division of Experimental Oncology/Unit of Urology, Urological Research Institute, IRCCS Ospedale San Raffaele, Milan, Italy; Vita-Salute San Raffaele University, Milan, Italy., Laboratory of Microbiology and Virology, IRCCS Ospedale San Raffaele, Milan, Italy., Division of Experimental Oncology/Unit of Urology, Urological Research Institute, IRCCS Ospedale San Raffaele, Milan, Italy., Department of Pathology, IRCCS Ospedale San Raffaele, Milan, Italy., Unit of Surgical Pathology, Department of Biomedical and Clinical Sciences "Luigi Sacco", University of Milan, Milan, Italy., Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Istituto Nazionale dei Tumori, Milan, Italy., Vita-Salute San Raffaele University, Milan, Italy; Laboratory of Microbiology and Virology, IRCCS Ospedale San Raffaele, Milan, Italy., Division of Experimental Oncology/Unit of Urology, Urological Research Institute, IRCCS Ospedale San Raffaele, Milan, Italy. Electronic address: .
PubMed http://www.ncbi.nlm.nih.gov/pubmed/32345542