The traditional use of medicinal plants in the management of sexual dysfunctions has a long history. This study investigated testicular dysfunction ameliorative effect of the methanolic roots extracts of Maytenus procumbens and Ozoroa paniculosa in a butanol-induced testicular dysfunction rat model. The rats in respective experimental groups were orally administered with the extract at 50 and 250 mg/kg bw, daily for 28 days. The cytotoxicity of the extracts was evaluated against HEK293, MCF-7, and HT29 cell lines. The extracts exhibited moderate (LC50 30.3-330.2 μg/mL) to weak (LC50 200.8-438.4 μg/mL) cytotoxicity level on the cancer and normal cells, respectively. While relatively lower serum testosterone levels and total sperm count along with decreased numbers of spermatogonia were noted in the untreated group, all these parameters were improved in the groups treated with the extracts at 250 mg/kg. Improved histomorphological changes of the testes were also observed when compared to the untreated group. While the extracts (at 250 mg/kg) increased serum reduced glutathione content and decreased malondialdehyde content, a relatively higher serum creatinine level was also observed in the treated animals group. The results indicate that the two plant extracts have potential to ameliorate testicular dysfunction.
Evidence-based complementary and alternative medicine : eCAM. 2017 Nov 19 [Epub]
Nkosinathi David Cele, Nonhlakanipho Felicia Sangweni, Rebamang Anthony Mosa, Dambudzo Penduka, Geraldine Genevive Lazarus, Moganavelli Singh, Godfrey Elijah Zharare, Andy Rowland Opoku
Department of Biochemistry and Microbiology, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, South Africa., School of Life Sciences, Biochemistry Department, University of KwaZulu-Natal, Durban 4000, South Africa., Department of Agriculture, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, South Africa.