There are no blood biomarkers for the diagnosis of renal cell carcinoma (RCC) in routine clinical use. We focused on the gene expression profile of peripheral blood cells obtained from RCC patients to discover novel biomarkers for RCC diagnosis. Using microarray analysis and quantitative verification, CXCL7 was shown to be significantly up-regulated in the peripheral blood cells of RCC patients. Importantly, aberrant CXCL7 expression was confirmed even in peripheral blood cells obtained from early stage (pT1a) RCC patients, and the expression level of CXCL7 in peripheral blood cells was a potential independent biomarker for the diagnosis of RCC by Receiver Operating Characteristic curve analysis (sensitivity of 70.0% and specificity of 64.0%, AUC = 0.722, a multiple logistic regression analysis, OR, 1.07; 95% CI, 1.03-1.11; P = 0.0004). Moreover, CXCL7 expression in peripheral blood cells significantly decreased after resection of the primary tumor. CXCL7 is more highly expressed in peripheral blood mononuclear cells than in neutrophils from both healthy controls and RCC patients. Interestingly, CXCL7 expression in peripheral blood mononuclear cells from healthy volunteers was significantly elevated following co-culture with RCC cells compared to those co-cultured with normal cells as a control. These results suggest that aberrant CXCL7 expression in peripheral blood cells is induced by RCC cells and may serve as a novel biomarker in the diagnosis of RCC. This article is protected by copyright. All rights reserved.
Cancer science. 2017 Oct 06 [Epub ahead of print]
Toshiro Kinouchi, Motohide Uemura, Cong Wang, Yu Ishizuya, Yoshiyuki Yamamoto, Takuji Hayashi, Kyosuke Matsuzaki, Wataru Nakata, Takahiro Yoshida, Kentaro Jingushi, Atsunari Kawashima, Takeshi Ujike, Akira Nagahara, Kazutoshi Fujita, Ryoichi Imamura, Yuko Ueda, Kaori Kitae, Kazutake Tsujikawa, Norio Nonomura
Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan., Department of Therapeutic Urologic Oncology, Osaka University Graduate School of Medicine, Osaka, Japan., Laboratory of Molecular and Cellular Physiology, Osaka University Graduate School of Pharmaceutical Sciences, Osaka, Japan.