BACKGROUND - We aimed to discover the potential microRNA (miRNA) targets and to explore the underlying molecular mechanisms of clear cell renal cell carcinoma (ccRCC).
METHODS - Microarray data of GSE16441 was downloaded from Gene Expression Omnibus database.
Differentially expressed genes (DEGs) and differentially expressed miRNAs between ccRCC tumors and matched non-tumor samples were analyzed. Target genes of differentially expressed miRNAs were screened. Besides, functional enrichment analysis of DEGs was performed, followed by protein-protein interaction (PPI) network construction and sub-module analysis. Finally, the integrated miRNA-DEGs network was constructed.
RESULTS - A total of 1758 up- and 2465 down-regulated DEGs were identified. Moreover, 15 up- and 12 down-regulated differentially expressed miRNAs were screened. The up-regulated DEGs were significantly enriched in pathways such as cell adhesion molecules and focal adhesion. Besides, the down-regulated DEGs were enriched in oxidative phosphorylation, and citrate cycle (TCA cycle). Moreover, eight sub-modules of PPI network were obtained. Totally, eight down-regulated miRNAs were identified to significantly regulate the DEGs and miRNA-200c that could regulate collagen, type V, alpha 2 (COL5A2) as well as COL5A3 was found to be the most significant. Additionally, 10 up-regulated miRNAs were identified to be significantly associated with the DEGs. Thereinto, miRNA-15a that could regulate ATPase, H(+) transporting, lysosomal 21 kDa, V0 subunit b (ATP6V0B) and miRNA-155 were found to be the most significant.
CONCLUSIONS - miRNA-200c that could regulate COL5A2 and COL5A3, miRNA-15a that could regulate ATP6V0B and miRNA-155 may play key roles in ccRCC progression. These miRNAs may be potential targets for ccRCC treatment.
Cancer cell international. 2016 Mar 03*** epublish ***
Yongsheng Chen, Lichen Teng, Wenhua Liu, Yan Cao, Dexin Ding, Wentao Wang, Hui Chen, Changfu Li, Ruihua An
Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Intensive Care Unit (ICU) Department, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086 Province Heilongjiang China., Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Department of Urology, Harbin Medical University Cancer Hospital, Harbin, 150040 Heilongjiang Province China., Department of Urology, The First Affiliated Hospital of Harbin Medical University, No.31 Youzheng Street, Harbin, 150001 Province Heilongjiang China.