To evaluate the collagen content in the bladder wall of men undergoing open prostate surgery.
From July 2014 to August 2016, men aged ≥ 50 years, presenting LUTS and undergoing open prostate surgery due to benign prostatic enlargement (BPE) or prostate cancer were prospectively enrolled. Preoperative assessment included validated questionnaires (IPSS and OAB-V8), lower urinary tract ultrasound, and urodynamics. Bladder biopsies were obtained during open prostatectomy for determination of collagen content (sirius red-picric acid stain; polarized light analysis). Collagen to smooth muscle ratio (C/M) in the detrusor was measured and its relationship with preoperative parameters was investigated. The level of significance was P < 0.05.
Thirty-eight consecutive patients were included in this pilot study. Mean age was 66.36 ± 6.44 years and mean IPSS was 11.05 ± 8.72 points. Men diagnosed with diabetes mellitus (DM2) were found to have higher collagen content in the bladder wall when compared to non-diabetic patients (17.71 ± 6.82% vs 12.46 ± 5.2%, respectively; P = 0.024). Reduced bladder compliance was also marker for higher collagen content (P = 0.042). Bladder outlet obstruction (BOO) was not a predictor of increased collagen deposition in the bladder wall (P = 0.75). Patients with PVR ≥ 200 mL showed a higher collagen to smooth muscle ratio in the bladder wall (P = 0.036).
DM2 and urodynamic parameters, such as increased PVR and reduced bladder compliance, were associated with higher collagen content in the bladder wall of men with LUTS.
Neurourology and urodynamics. 2017 Sep 25 [Epub ahead of print]
Marcio A Averbeck, Nelson G De Lima, Gabriela A Motta, Lauro F Beltrao, Nury J Abboud Filho, Clarice P Rigotti, William N Dos Santos, Steven K J Dos Santos, Luis F B Da Silva, Ernani L Rhoden
Postgraduate in Health Sciences at Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre, Brazil., Hospital Nossa Senhora dos Navegantes, Torres, Brazil., Urology Residency at Santa Casa Hospital Complex, Porto Alegre, Brazil., Santa Casa Hospital Complex, Porto Alegre, Brazil.