The utility of urine-circulating miRNAs for detection of prostate cancer.

In this paper, the utility of urine-circulating microRNAs (miRNAs) as the potential biomarker of prostate cancer (PCa), the second most prevalent male cancer worldwide, was evaluated.

Cancerous (N=56) and non-cancerous (N=16) prostate tissues were analysed on TaqMan Low Density Array, with the initial screening of 754 miRNAs in a subset of the samples. The abundance of selected miRNAs was analysed in urine specimens from two independent cohorts of patients with PCa (N=215 overall), benign prostatic hyperplasia (BPH; N=23), and asymptomatic controls (ASC; N=62) by means of quantitative reverse transcription PCR.

Over 100 miRNAs were found deregulated in PCa as compared with non-cancerous prostate tissue. After thorough validation, four miRNAs were selected for the analysis in urine specimens. The abundance of miR-148a and miR-375 in urine was identified as specific biomarkers of PCa in both cohorts. Combined analysis of urine-circulating miR-148a and miR-375 was highly sensitive and specific for PCa in both cohorts (AUC=0.79 and 0.84) and strongly improved the diagnostic power of the PSA test (AUC=0.85, cohort PCa1), including the grey diagnostic zone (AUC=0.90).

Quantitative measurement of urine-circulating miR-148a and miR-375 can serve as the non-invasive tool for sensitive and specific detection of PCa.British Journal of Cancer advance online publication 4 August 2016; doi:10.1038/bjc.2016.233 www.bjcancer.com.

British journal of cancer. 2016 Aug 04 [Epub ahead of print]

Kristina Stuopelyte, Kristina Daniunaite, Arnas Bakavicius, Juozas R Lazutka, Feliksas Jankevicius, Sonata Jarmalaite

Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Sauletekio Avenue 7, Vilnius LT-10222, Lithuania., Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Sauletekio Avenue 7, Vilnius LT-10222, Lithuania., Urology Centre, Vilnius University, Santariskiu 2, Vilnius LT-08661, Lithuania., Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Sauletekio Avenue 7, Vilnius LT-10222, Lithuania., Urology Centre, Vilnius University, Santariskiu 2, Vilnius LT-08661, Lithuania., Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Sauletekio Avenue 7, Vilnius LT-10222, Lithuania.