Anticancer effect of docetaxel induces apoptosis of prostate cancer via the cofilin-1 and paxillin signaling pathway.

Prostate cancer is a common multiple malignant tumor occurring in males. Prostate cancer mortality is the 2nd most common of all tumor types in Western countries and the mortality of morbidity is 13% in the USA.

The present study aimed to investigate the anticancer effect of docetaxel on inducing the apoptosis of prostate cancer via the cofilin‑1 and paxillin signaling pathway. Treatment with docetaxel (1‑50 nM) disposed the human LNCaP prostate cancer cells for 24 h. Cell growth and cytotoxicity were subsequently measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase assay, respectively. Docetaxel-induced cell death was analyzed using flow cytometric and caspase-3 assays. Reverse transcription‑quantitative polymerase chain reaction analysis was used to detect the gene expression of cofilin‑1 and western blots were used to determine the protein expression of paxillin. Treatment with docetaxel inhibited cell growth, promoted cytotoxicity, activated apoptosis and increased caspase‑3 activity in the LNCaP cells. Notably, administration of docetaxel reduced the gene expression of cofilin‑1 and the protein expression of paxillin in the LNCaP cells. Additionally, knockdown of cofilin‑1 advanced the anticancer effect of docetaxel against LNCaP cells through suppression of the paxillin pathway. The present findings demonstrated that the anticancer effect of docetaxel induces the apoptosis of prostate cancer via the suppression of the cofilin‑1 and paxillin signaling pathways, which will assist in setting a stage for the clinical treatment of prostate cancer.

Molecular medicine reports. 2016 Mar 18 [Epub ahead of print]

Pan Xiao, Tianjia Ma, Chunwen Zhou, Yang Xu, Yuqiang Liu, Huaiqiang Zhang

Department of Urology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China., Department of Urology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China., Department of Urology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China., Department of Urology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China., Department of Urology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China., Department of Urology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.

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