Intracellular kinetics of the androgen receptor shown by multimodal Image Correlation Spectroscopy (mICS).

The androgen receptor (AR) pathway plays a central role in prostate cancer (PCa) growth and progression and is a validated therapeutic target. In response to ligand binding AR translocates to the nucleus, though the molecular mechanism is not well understood.

We therefore developed multimodal Image Correlation Spectroscopy (mICS) to measure anisotropic molecular motion across a live cell. We applied mICS to AR translocation dynamics to reveal its multimodal motion. By integrating fluorescence imaging methods we observed evidence for diffusion, confined movement, and binding of AR within both the cytoplasm and nucleus of PCa cells. Our findings suggest that in presence of cytoplasmic diffusion, the probability of AR crossing the nuclear membrane is an important factor in determining the AR distribution between cytoplasm and the nucleus, independent of functional microtubule transport. These findings may have implications for the future design of novel therapeutics targeting the AR pathway in PCa.

Scientific reports. 2016 Mar 03*** epublish ***

Chi-Li Chiu, Katherin Patsch, Francesco Cutrale, Anjana Soundararajan, David B Agus, Scott E Fraser, Daniel Ruderman

Center for Applied Molecular Medicine, University of Southern, California, USA., Center for Applied Molecular Medicine, University of Southern, California, USA., Translational Imaging Center, University of Southern, California, USA., Center for Applied Molecular Medicine, University of Southern, California, USA., Center for Applied Molecular Medicine, University of Southern, California, USA., Translational Imaging Center, University of Southern, California, USA., Center for Applied Molecular Medicine, University of Southern, California, USA.

E-Newsletters

Newsletter subscription

Free Daily and Weekly newsletters offered by content of interest

The fields of GU Oncology and Urology are rapidly advancing. Sign up today for articles, videos, conference highlights and abstracts from peer-review publications by disease and condition delivered to your inbox and read on the go.

Subscribe