An electrochemical biosensor to simultaneously detect VEGF and PSA for early prostate cancer diagnosis based on graphene oxide/ssDNA/PLLA nanoparticles.

Early diagnosis of prostate cancer (PCa) is critical for the prevention of metastasis and for early treatment; therefore, a simple and accurate device must be developed for this purpose. In this study, we reported a novel fabrication method for producing a dual-modality biosensor that can simultaneously detect vascular endothelial growth factor (VEGF) and prostate-specific antigen (PSA) in human serum for early diagnosis of PCa.

This biosensor was constructed by coating graphene oxide/ssDNA (GO-ssDNA) on an Au-electrode for VEGF detection, and incorporated with poly-L-lactide nanoparticles (PLLA NPs) for signal amplification and PSA detection. The results showed that this biosensor has wide liner detection ranges (0. 05-100ng/mL for VEGF and 1-100ng/mL for PSA), as well as high levels of sensitivity and selectivity (i. e. , resisting interference from external factors, such as glucose, ascorbic acid human serum protein, immunoglobulin G, and immunoglobulin M), and demonstrated a high correlation with an enzyme-linked immunosorbent assay for sample detection in patients. Therefore, this biosensor could be utilized for early clinical diagnosis of PCa in the future.

Biosensors & bioelectronics. 2016 Jan 29 [Epub ahead of print]

Lung-Hsuan Pan, Shin-Hung Kuo, Tzu-Yang Lin, Chih-Wen Lin, Po-Yu Fang, Hung-Wei Yang

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. , Department of Electrical Engineering, National Cheng Kung University, Tainan 70101, Taiwan. , Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. , Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan. , School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA. , Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.

PubMed