The combination of DNA ploidy status and PTEN/6q15 deletions provides strong and independent prognostic information in prostate cancer.

PURPOSE - Aberrant DNA content has been discussed as a potential prognostic feature in prostate cancer.

EXPERIMENTAL DESIGN - We analyzed the clinical significance of DNA ploidy in combination with prognostic relevant deletions of PTEN and 6q15 in 3,845 prostate cancers.

RESULTS - The DNA status was diploid in 67. 8%, tetraploid in 25. 6% and aneuploid in 6. 8% of tumors, and deletions of PTEN and 6q15 occurred in 17. 8% and 20. 3% of tumors. Abnormal DNA content and deletions were linked to high Gleason score, advanced tumor stage, and positive nodal stage (p<0. 0001 each). The risk of PSA recurrence increased from diploid to tetraploid and from tetraploid to aneuploid DNA status (p<0. 0001 each). However, 40% of patients with Gleason score ≥4+4 and 55% of patients with PSA recurrence had diploid cancers. This fraction decreased to 21% (Gleason ≥4+4) and 29% (PSA recurrence) if PTEN and/or 6q deletion data were added to ploidy data to identify cancers with an aberrant DNA status. The significance of combining both deletions and ploidy was further demonstrated in a combined recurrence analysis. Presence of deletions increased the risk of PSA recurrence in diploid (p<0. 0001), tetraploid (p<0. 0001), and aneuploid cancers (p=0. 0049), and the combination of ploidy data and deletions provided clinically relevant information beyond the CAPRA-S nomogram. Multivariate modeling including preoperatively and postoperatively available parameters identified the "combined DNA status" as a strong independent predictor of poor patient outcome.

CONCLUSIONS - The combinatorial DNA content analysis involving general (ploidy) and specific events (deletions) has the potential for clinical utility in prostate cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research. 2016 Jan 26 [Epub ahead of print]

Maximilian Lennartz, Sarah Minner, Sophie Brasch, Hilko Wittmann, Leonard Paterna, Katja Angermeier, Eray Öztürk, Rami Shihada, Mingu Ruge, Martina Kluth, Christina Koop, Waldemar Wilczak, Till Krech, Patrick Lebok, Corinna Wittmer, Hans Heinzer, Thomas Steuber, Meike Adam, Hartwig Huland, Markus Graefen, Alexander Haese, Ronald Simon, Guido Sauter, Thorsten Schlomm

Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg Eppendorf. , Institute of Pathology, Universtity Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf. , Martini-Clinic, Prostate Cancer Center, University Medical Center Hamburg-Eppendorf. , Martini-Clinic, Prostate Cancer Center, University Medical Center Hamburg-Eppendorf. , Martini-Clinic, Prostate Cancer Center, University Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg Eppendorf. , Institute of Pathology, University Medical Center Hamburg Eppendorf. , Martini-Clinic Prostate Cancer Center, Universtity Medical Center Hamburg-Eppendorf. , Institute of Pathology, University Medical Center Hamburg-Eppendorf Pathology, University Medical Centre Hamburg-Eppendorf. , Prostate Cancer Center, University Medical Centre Hamburg-Eppendorf.

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