Inhibition of Hedgehog-Signaling Driven Genes in Prostate Cancer Cells by Sutherlandia frutescens Extract.

Sutherlandia frutescens (L) R. Br. (Sutherlandia) is a South African botanical that is traditionally used to treat a variety of health conditions, infections and diseases, including cancer. We hypothesized Sutherlandia might act through Gli/ Hedgehog (Hh)-signaling in prostate cancer cells and used RNA-Seq transcription profiling to profile gene expression in TRAMPC2 murine prostate cancer cells with or without Sutherlandia extracts.

We found 50% of Hh-responsive genes can be repressed by Sutherlandia ethanol extract, including the canonical Hh-responsive genes Gli1 and Ptch1 as well as newly distinguished Hh-responsive genes Hsd11b1 and Penk.

PloS one. 2015 Dec 28*** epublish ***

Yuan Lu, Nicholas Starkey, Wei Lei, Jilong Li, Jianlin Cheng, William R Folk, Dennis B Lubahn

Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America. , Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America. , MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America. , MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America. , MU Center for Botanical Interaction Studies, University of Missouri, Columbia, MO 65211, United States of America. , Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America. , Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States of America.

PubMed