The potential use of urinary nucleic acids as diagnostic markers in prostate cancer (PCa) was evaluated. Ninety-five urine samples and 234 prostate tissue samples from patients with PCa and benign prostatic hyperplasia (BPH) were analyzed.
Micro-array analysis was used to identify candidate genes, which were verified by the two-gene expression ratio and validated in tissue mRNA and urinary nucleic acid cohorts. Real-time quantitative polymerase chain reaction (qPCR) was used to measure urinary nucleic acid levels and tissue mRNA expression. The TSPAN13-to-S100A9 ratio was selected to determine the diagnostic value of urinary nucleic acids in PCa (P = 0. 037) and shown to be significantly higher in PCa than in BPH in the mRNA and nucleic acid cohort analyses (P < 0. 001 and P = 0. 013, respectively). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0. 898 and 0. 676 in tissue mRNA cohort and urinary nucleic acid cohort, respectively. The TSPAN13-to-S100A9 ratio showed a strong potential as a diagnostic marker for PCa. The present results suggest that the analysis of urine supernatant can be used as a simple diagnostic method for PCa that can be adapted to the clinical setting in the future.
Journal of Korean medical science. 2015 Nov 30 [Epub]
Chunri Yan, Ye-Hwan Kim, Ho Won Kang, Sung Phil Seo, Pildu Jeong, Il-Seok Lee, Dongho Kim, Jung Min Kim, Yung Hyun Choi, Sung-Kwon Moon, Seok Joong Yun, Wun-Jae Kim
Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. , Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. , Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. , Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. , Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. , Bio-Medical Science Co. Ltd, Daejeon, Korea. , Bio-Medical Science Co. Ltd, Daejeon, Korea. , Nucleic Acid Research Center, Inc. , Daejeon Oriental Hospital of Daejeon University, Daejeon, Korea. , Department of Biochemistry, Dong-Eui University, Busan, Korea. , Department of Food and Biotechnology, Chung-Ang University, Seoul, Korea. , Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. , Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea.