VPAC1 Targeted (64)Cu-TP3805 PET Imaging of Prostate Cancer: Preliminary Evaluation in Man.

To evaluate (64)Cu-TP3805 as a novel biomolecule, to PET image prostate cancer (PC), at the onset of which VPAC1, the superfamily of G-protein coupled receptors, is expressed in high density on PC cells, but not on normal cells.

25 patients undergoing radical prostatectomy were PET/CT imaged preoperatively with (64)Cu-TP3805. Standardized uptake values (SUVmax) were determined, malignant lesions (SUV > 1. 0) counted, and compared with histologic findings. Whole mount pathology slides from 6 VPAC1 PET imaged patients, 3 BPH patients, one malignant and one benign lymph node underwent digital autoradiography (DAR) after (64)Cu-TP3805 incubation and compared to H&E stained slides.

In 25 patient PET imaging, 212 prostate gland lesions had SUVmax > 1. 0 vs. 127 lesions identified by histology of biopsy tissues. The status of the additional 85 PET identified prostate lesions remains to be determined. In 68 histological slides from 6 PET imaged patients, DAR identified 105/107 PC foci, 19/19 HGPIN, and ejaculatory ducts and verumontanum involved with cancer. Additionally, DAR found 9 PC lesions not previously identified histologically. The positive and negative lymph nodes were correctly identified and in 3/3 BPH patients and 5/5 cysts, DAR was negative.

This feasibility study demonstrated that (64)Cu-TP3805 delineates PC in vivo and ex vivo, provided normal images for benign masses, and is worthy of further studies.

Urology. 2015 Oct 28 [Epub ahead of print]

Sushil Tripathi, Edouard J Trabulsi, Leonard Gomella, Sung Kim, Peter McCue, Charles Intenzo, Ruth Birbe, Ashish Gandhe, Pardeep Kumar, Mathew Thakur

Thomas Jefferson University, Department of Radiology. , Thomas Jefferson University, Department of Urology; Thomas Jefferson University, Sidney Kimmel Cancer Center. , Thomas Jefferson University, Department of Urology; Thomas Jefferson University, Sidney Kimmel Cancer Center. , Thomas Jefferson University, Department of Radiology. , Thomas Jefferson University, Department of Pathology; Thomas Jefferson University, Sidney Kimmel Cancer Center. , Thomas Jefferson University, Department of Radiology. , Thomas Jefferson University, Department of Pathology; Thomas Jefferson University, Sidney Kimmel Cancer Center. , Thomas Jefferson University, Department of Radiology. , Thomas Jefferson University, Department of Radiology. , Thomas Jefferson University, Department of Radiology; Thomas Jefferson University, Sidney Kimmel Cancer Center.  

PubMed