The current treatments fail to provide satisfactory cure for aggressive prostate cancers (PCs). Hence, further comprehension of PC metastasis is highly appreciated for improving the levels of therapy.
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We have previously shown that Adiponectin reduces the levels of vascular endothelial growth factor A (VEGF-A) in PCs to suppress tumor-associated neovascularization, possibly through AMPK/mTor signaling Here, we studied the regulation of Adiponectin signaling in PCs
We analyzed the levels and correlation of Adiponectin receptor 1 (AdipoR1) and microRNA-323 (miR-323) in the PC specimen, compared to the paired normal prostate tissue We analyzed the binding of miR-323 to the 3'UTR of AdipoR1 mRNA and its effects on AdipoR1 translation by bioinformatics analysis and by luciferase-reporter assay, respectively We modified miR-323 levels in PC cells, and examined the effects on the expression of AdipoR1 and VEGF-A, as well as on vessel formation in a human umbilical vein endothelial cells (HUVECs) transwell collagen gel assay
We detected significantly lower levels of AdipoR1 and significantly higher levels of miR-323 in PC specimen Moreover, the levels of AdipoR1 and miR-323 are inversely correlated Moreover, miR-323 was found to bind to the 3'UTR of AdipoR1 mRNA to inhibit its translation Overexpression of miR-323 in PC cells decreased AdipoR1 protein levels, whereas inhibition of miR-323 increased AdipoR1 protein levels, without affecting AdipoR1 transcripts Moreover, overexpression of miR-323 increased the levels of VEGF-A and the vessel formation by HUVECs, while inhibition of miR-323 decreased the levels of VEGF-A and the vessel formation by HUVECs
Our data demonstrate that miR-323 may increase VEGF-A-mediated cancer vascularization in PC cells through AdipoR1 suppression © 2015 S Karger AG, Basel
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015 Jul 06 [Epub ahead of print]
Qiruo Gao, Xudong Yao, Junhua Zheng
Department of Urology, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai, China