Detection and phenotyping of circulating tumor cells in high-risk localized prostate cancer - Abstract

BACKGROUND: In this study, we aimed to determine the feasibility of identifying CTCs in patients with HRLPC, using a modified isolation procedure using the CellSearch (Veridex) platform, and to assess the expression of stem cell and epithelial-mesenchymal transition (EMT) markers on the CTCs.

PATIENTS AND METHODS: Thirty-five patients with HRLPC who had chosen prostatectomy for definitive management were prospectively identified. After obtaining consent, four 30-mL blood draws were performed, 2 before surgery and 2 after surgery. The CTC-containing fraction was Ficoll-purified and transferred to a CellSave (Veridex) tube containing dilution buffer before standard enumeration using the CellSearch system. Loss of E-cadherin expression, a marker of EMT, and CD133, a putative prostate cancer stem cell marker, were characterized using the open channel of the CellSearch platform. CTC fragments were also enumerated.

RESULTS: Using the modified methodology, CTCs were detectable in 49% of patients before surgery. Although no correlation between CTC count and biochemical recurrence (BR) was observed, the percentages of CD133 and E-cadherin-positive CTC fragments were associated with BR at 1 year.

CONCLUSION: Our results suggest that further research into the development of CTCs as prognostic biomarkers in HRLPC is warranted.

Written by:
Pal SK, He M, Wilson T, Liu X, Zhang K, Carmichael C, Torres A, Hernandez S, Lau C, Agarwal N, Kawachi M, Yen Y, Jones JO.   Are you the author?
Department of Medical Oncology and Experimental Therapeutics, City of Hope Comprehensive Cancer Center, Duarte, CA; Department of Molecular Pharmacology, City of Hope Comprehensive Cancer Center, Duarte, CA; Department of Urology, City of Hope Comprehensive Cancer Center, Duarte, CA; Department of Biostatistics, City of Hope Comprehensive Cancer Center, Duarte, CA; Clinical Trials Office, City of Hope Comprehensive Cancer Center, Duarte, CA; Department of Medical Oncology, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT.  

Reference: Clin Genitourin Cancer. 2014 Sep 22. pii: S1558-7673(14)00207-9.
doi: 10.1016/j.clgc.2014.08.014


PubMed Abstract
PMID: 25450039

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