Genomic landscape of early-stage prostate adenocarcinoma in Mexican patients: an exploratory study.

Health disparities have been highlighted among patient with prostate adenocarcinoma (PRAD) due to ethnicity. Mexican men present a more aggressive disease than other patients resulting in less favorable treatment outcome. We aimed to identify the mutational landscape which could help to reduce the health disparities among minority groups and generate the first genomics exploratory study of PRAD in Mexican patients.

Paraffin-embedded formalin-fixed tumoral tissue from 20 Mexican patients with early-stage PRAD treated at The Instituto Nacional de Cancerología, Mexico City from 2017 to 2019 were analyzed. Tumoral DNA was prepared for whole exome sequencing, the resulting files were mapped against h19 using BWA-MEM. Strelka2 and Lancet packages were used to identify single nucleotide variants (SNV) and insertions or deletions. FACETS was used to determine somatic copy number alterations (SCNA). Cancer Genome Interpreter web interface was used to determine the clinical relevance of variants.

Patients were in an early clinical stage and had a mean age of 59.55 years (standard deviation [SD]: 7.1 years) with 90% of them having a Gleason Score of 7. Follow-up time was 48.50 months (SD: 32.77) with recurrences and progression in 30% and 15% of the patients, respectively. NUP98 (20%), CSMD3 (15%) and FAT1 (15%) were the genes most frequently affected by SNV; ARAF (75%) and ZNF419 (70%) were the most frequently affected by losses and gains SNCA's. One quarter of the patients had mutations useful as biomarkers for the use of PARP inhibitors, they comprise mutations in BRCA, RAD54L and ATM. SBS05, DBS03 and ID08 were the most common mutational signatures present in this cohort. No associations with recurrence or progression were identified.

This pilot study reveals the mutational landscape of early-stage prostate adenocarcinoma in Mexican men, providing a first approach to understand the mutational patterns and actionable mutations in early prostate cancer can inform personalized treatment approaches and reduce the underrepresentation in genomic cancer studies.

Discover oncology. 2024 Aug 28*** epublish ***

Dennis Cerrato-Izaguirre, Jonathan González-Ruíz, José Diaz-Chavez, Andrea Ramírez, Anna Scavuzzo, Miguel A Jimenez, Carlo Cortés-González, Jairo A Rubio, María D Pérez-Montiel, Claudia M García-Cuellar, Luis A Herrera, Yesennia Sánchez-Pérez, Felipe Vaca-Paniagua, Salim Barquet-Muñoz, David Cantu-de-Leon, Promita Bose, Diddier Prada

Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Mexico City, Mexico., Unidad de Apoyo Molecular para la Investigación Clínica, Instituto Nacional de Cancerología, Mexico City, Mexico., Departamento de Urología, Instituto Nacional de Cancerología, Mexico City, Mexico., Departamento de Patología, Instituto Nacional de Cancerología, Mexico City, Mexico., Dirección de Investigación, Instituto Nacional de Cancerología, Mexico City, Mexico., Laboratorio Nacional en Salud, Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, Facultad de Estudios Superiores Iztacala, UNAM, Tlalnepantla, Mexico., Department of Environmental Health Sciences, Mailman School of Public Health Columbia University, New York, NY, USA., Institute for Health Equity Research, Icahn School of Medicine at Mount Sinai, New York City, NY, USA. .