Our study investigated the expression levels of miR-1231 in prostate cancer tissues and cell lines and explored its potential prognostic significance as well as its functional effects on prostate cancer cells.
miR-1231 expression levels were detected in prostate cancer tissues and prostate cancer cell lines using qRT-PCR. The prognostic significance of miR-1231 in prostate cancer was assessed using the Kaplan-Meier curve and Cox regression analyses. Evaluation of the impact of ectopic expression or inhibition of miR-1231 on aggressive tumor behavior (cellular proliferation, migration, and invasion) of prostate cancer cell lines involved the CCK-8 and Transwell assays.
miR-1231 expression was downregulated in both prostate cancer tissues and cell lines. Downregulation of miR-1231 was significantly associated with lymph node metastasis, higher TNM stage, higher clinical stage, and shorter overall survival. The expression of miR-1231 was predicted as a prognostic factor for prostate cancer patients. Additionally, we found overexpression of miR-1231 suppressed proliferation, migration, and invasion of prostate cancer cell linesin vitro. EGFR was a direct target of miR-1231.
Taken together, our results indicate that miR-1231 expression plays a tumor-suppressive role in prostate cancer. It is downregulated in prostate cancer and may suppress prostate cancer cell proliferation, migration, and invasion by targeting EGFR, and it may be a prognostic biomarker and potential therapeutic strategy for prostate cancer treatment in the future.
Oncology research and treatment. 2019 Dec 10 [Epub ahead of print]
Yitao Wang, Qingling Zhang, Baowei Guo, Jiao Feng, Dan Zhao
Department of Laboratory, Weihai Municipal Hospital, Weihai, China., Department of Labor Union, Yidu Central Hospital of Weifang, Weifang, China., Department of Urology Surgery, Yidu Central Hospital of Weifang, Weifang, China, ., Department of Urology Surgery, Yidu Central Hospital of Weifang, Weifang, China.