Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs

MicroRNAs (miRNAs) in urine are examined as potential biomarkers. We examined the urine samples from 70 individuals (45 males, 25 females, mean age 65 years, range 20-84 years). Of the urine donors, 15 were healthy volunteers, 5 were patients with non-cancer diseases, 50 were patients with different stages of bladder cancer. To examine the spectrum of miRNAs in the cell-free fraction of urine, TaqMan Human miRNA Array Card A v.2.1 was used. A set of 30 miRNAs were found that are constantly present in urine supernatants independently of sex, age and health status of the subjects. We compared this set with miRNAs found in plasma, expressed in kidney and genito-urinary tract. Our results indicate that some miRNA could be transferred from the circulation into urine.

Advances in experimental medicine and biology. 2016 Jan [Epub]

Eva Pazourkova, Sarka Pospisilova, Iveta Svobodova, Ales Horinek, Antonin Brisuda, Viktor Soukup, Jan Hrbacek, Otakar Capoun, Jaroslav Mares, Tomas Hanus, Marek Babjuk, Marie Korabecna

Department of Nephrology, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic., Department of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic., Department of Urology, 2nd Faculty of Medicine, Charles University and University Hospital Motol in Prague, Prague, Czech Republic., Department of Urology, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic., Department of Biology and Medical Genetics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic., Department of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General Faculty Hospital in Prague, Prague, Czech Republic. .