LINC00312 inhibits the migration and invasion of bladder cancer cells by targeting miR-197-3p

To investigate the influence of the long non-coding RNA LINC00312 on bladder cancer (BC) cell invasion and metastasis by targeting miR-197-3p. BC and corresponding adjacent tissues were collected. LINC00312 and miR-197-3p were measured, and their correlation was detected through quantitative real-time PCR (qRT-PCR). BC cell line T24 was transfected and grouped (five groups) according to different transfection conditions. A scratch test was applied to analyze cell migration, and a Transwell assay was used to test cell invasion ability. Western blotting was to measure matrix metalloproteinase (MMP)-2, MMP-9, and the tissue inhibitor of metalloproteinase 2 (TIMP2) protein levels. qRT-PCR indicated that LINC00312 expression was lower but miR-197-3p expression was higher in BC tissues compared with adjacent tissues; LINC00312 was negatively correlated with miR-197-3p. The migration test revealed that the downregulation of miR-197-3p and overexpression of LINC00312 inhibited cell migration and invasion abilities, while the overexpression of miR-197-3p and the upregulation of LINC00312 promoted cell migration and invasion. BC cells with downregulated miR-197-3p or upregulated LINC00312 had low MMP-2 and MMP-9 levels but high TIMP2. LINC00312 inhibited BC cell invasion and metastasis through mediating miR-197-3p.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016 Sep 08 [Epub ahead of print]

Yun-Yan Wang, Zi-Yu Wu, Gong-Cheng Wang, Kun Liu, Xiao-Bing Niu, Shuo Gu, Jun-Song Meng

Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China., Department of Urology, Huai'an Hospital affiliated of Xuzhou Medical College, Huai'an, 223002, People's Republic of China., Department of Urology, Huai'an First People's Hospital affiliated with Nanjing Medical University, No. 6 Beijing West Road, 223300, Huai'an, Jiangsu Province, People's Republic of China. .