BACKGROUND - Hyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes. Resveratrol and quercetin are red wine polyphenols with known beneficial cardiovascular properties, including an antioxidant capacity.
This study evaluated whether resveratrol and/or quercetin could prevent in vitro-induced diabetic changes in neurogenic and vascular relaxant responses of mouse arteries and corpora cavernosa.
METHODS - Isometric tension of isolated aorta, mesenteric arteries and corpora cavernosa was measured using organ bath systems. Diabetic conditions were mimicked in vitro by co-incubating the tissues for 2 h with high glucose (HG, 30 mM) and MGO (120 µM).
RESULTS - The presence of HG and MGO significantly blunted acetylcholine (Ach)-induced relaxations in corpora cavernosa and mesenteric arteries but not in aorta. Electrical field stimulated (EFS) responses of corpora cavernosa were also significantly inhibited by these diabetic conditions. In corpora cavernosa 2 h co-incubation with resveratrol (30 µM) or quercetin (30 µM) significantly attenuated HG and MGO-induced deficits in Ach- and EFS-responses.
CONCLUSIONS - Our study demonstrates that in mouse arteries, HG and MGO rather affect endothelium derived hyperpolarizing factor-mediated than nitric oxide (NO)-mediated relaxations. In corpora cavernosa HG and MGO interfere with NO release. Resveratrol and quercetin protect mouse corpora cavernosa from diabetic-induced damage to NO-mediated relaxant responses. This might rely on their antioxidant capacity.
Cardiovascular diabetology. 2016 Mar 18*** epublish ***
Charlotte Boydens, Bart Pauwels, Laura Vanden Daele, Johan Van de Voorde
Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium., Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium., Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium., Department of Pharmacology, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium.