Diagnostic and prognostic value of tissue and circulating levels of Ephrin-A2 in prostate cancer

Ephrin-A2, a member of the Eph/ephrin family, is associated with tumorigenesis and tumor progression. This study aimed to assess the diagnostic and prognostic value of both serum and tissue levels of Ephrin-A2 in prostate cancer (PCa) management.

One hundred and forty-five frozen prostate tissues, 55 paraffin-embedded prostate tissues, 88 serum samples, and seven prostate cell lines (RWPE-1, LNCaP, LNCaP-LN3, PC-3, PC-3M, PC-3M-LN4, and DU145) were examined via quantitative reverse transcription-PCR (qRT-PCR), immunohistochemistry, enzyme-linked immunosorbent assay, and western blotting. Induced Ephrin-A2 messenger RNA (mRNA) or protein expression was detected in 8. 6 % (5/58) benign prostatic hyperplasia (BPH), 59. 8 % (52/87) PCa, and five prostate cancer cell lines. Ephrin-A2 immunostaining was present in 6. 7 % (1/15) patients with BPHs and 62. 5 % (25/40) clinically localized PCa. Accordingly, serum Ephrin-A2 was significantly higher in PCa patients compared to those in the BPH patients and controls (P < 0. 001). The expression of Ephrin-A2 was higher in tumor patients with an elevated Gleason score or T3-T4 staging. Ephrin-A2 expression was correlated with Ki-67 expression in PCa patients, both at the gene scale and protein level. Our data indicate that Ephrin-A2 is a potential diagnostic and prognostic biomarker and a promising molecular therapeutic target to attenuate prostate cancer progression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015 Nov 11 [Epub ahead of print]

Shibao Li, Zhiyuan Wu, Yuming Chen, Zhihua Kang, Hua Wang, Ping He, Xinju Zhang, Tingting Hu, Qunfeng Zhang, Yanqun Cai, Xiao Xu, Ming Guan

Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital North, Fudan University, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Department of Laboratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China. , Central Laboratory, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. , Central Laboratory, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China. , Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.

PubMed