Exosomes as biomarker enriched microvesicles: Characterization of exosomal proteins derived from a panel of prostate cell lines with distinct AR phenotypes - Abstract

Prostate cancer is the leading type of cancer diagnosed in men. In 2010, approximately 217,730 new cases of prostate cancer were reported in the United States. Prompt diagnosis of the disease can substantially improve its clinical outcome. Improving capability for early detection, as wellas developing new therapeutic targets in advanced disease are research priorities that will ultimately lead to better patient survival.Eukaryotic cells secrete proteins via distinct regulated mechanisms which are either ER/Golgidependent or microvesicle mediated. The release of microvesicles has been shown to provide a novel mechanism for intercellular communication. Exosomes are nanometer sized cup-shaped membrane vesicles which are secreted from normal and cancerous cells. They are present in various biological fluids and are rich in characteristic proteins. Exosomes may thus have potential both in facilitating early diagnosis via less invasive procedures or be candidates for novel therapeutic approaches for castration resistance prostate cancer.Since exosomes have been shown previously to have a role in cell-cell communication in thelocal tumour microenvironment, conferring activation of numerous survival mechanisms, wecharacterized constitutive lipids, cholesterol and proteins from exosomes derived from sixprostate cell lines and tracked their uptake in both cancerous and benign prostate cell lines respectively. Our comprehensive proteomic and lipidomic analysis of prostate derived exosomes could provide insight for future work on both biomarkers and therapeutic targets for the treatment of for prostate cancer.

Written by:
Hosseini-Beheshti E, Pham S, Adomat H, Li N, Guns ES   Are you the author?
The Vancouver Prostate Centre, University of British Columbia, Canada

Reference: Mol Cell Proteomics. 2012 Jun 21. [Epub ahead of print]
doi: 10.1074/mcp.M111.014845

PubMed Abstract
PMID: 22723089