In this work, we investigated the effects of red orange and lemon extract (RLE) on ochratoxin A (OTA)-induced nephrotoxicity. In particular, we analyzed the change in renal function and oxidative stress in Sprague-Dawley rats treated with OTA (0. 5 mg/kg body weight, b.w.) and with RLE (90 mg/kg b.w.) by oral administration. After OTA treatment, we found alterations of biochemical and oxidative stress parameters in the kidney, related to a severe decrease of glomerular filtration rate. The RLE treatment normalized the activity of antioxidant enzymes and prevented the glomerular hyperfiltration. Histopathological examinations revealed glomerular damages and kidney cortex fibrosis in OTA-rats, while we observed less severe fibrosis in OTA plus RLE group. Then, we demonstrated that oxidative stress could be the cause of OTA renal injury and that RLE reduces this effect.
Journal of cellular physiology. 2020 Jan 03 [Epub ahead of print]
Sara Damiano, Valentina Iovane, Caterina Squillacioti, Nicola Mirabella, Francesco Prisco, Andrea Ariano, Margherita Amenta, Antonio Giordano, Salvatore Florio, Roberto Ciarcia
Department of Veterinary Medicine and Animal Productions, University of Naples, Napoli, Italy., Department of Pharmacy, University of Salerno, Fisciano, Italy., Council for Agricultural Research and Economics, Research Centre for Olive, Citrus and Tree Fruit, Acireale, Italy., Department of Medical Biotechnologies, University of Siena, Siena, Italy.