Characterization of N-cadherin unbinding properties in non-malignant (HCV29) and malignant (T24) bladder cells - Abstract

Laboratory of Biophysical Microstudies, The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Kraków, Poland.

www.ifj.edu.pl

 

The expression of N-cadherin, characteristic of various cancers, very often leads to changes in the cells' adhesive properties. Thus, we sought to find out if N-cadherin expressed in various, but cancer-related cells, differs in its functional properties that could contribute to variations in cells' phenotypes. In our work, measurements of an unbinding force of a single N-cadherin molecule, probed with the same antibody both on a surface of living non-malignant (HCV29) and malignant cells (T24) of bladder cancer, were carried out with the use of an atomic force microscopy. The results show the enhanced N-cadherin level in T24 malignant cells (8.7% vs. 3.6% obtained for non-malignant one), confirmed by the Western blot and the immunohistochemical staining. The effect was accompanied by changes in unbinding properties of an individual N-cadherin molecule. Lower unbinding force values (26.1 ± 7.1 pN) in non-malignant cells reveal less stable N-cadherin complexes, as compared to malignant cells (61.7 ± 14.6 pN). This suggests the cancer-related changes in a structure of the binding site of the antibody, located at the extracellular domain of N-cadherin.

Written by:
Lekka M, Gil D, Dąbroś W, Jaczewska J, Kulik AJ, Lekki J, Stachura Z, Stachura J, Laidler P.   Are you the author?

Reference: J Mol Recognit. 2011 Aug;24(5):833-42.
doi: 10.1002/jmr.1123

PubMed Abstract
PMID: 21812057

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