Gamma/Delta T-Cells Enhance Carboplatin-induced Cytotoxicity Towards Advanced Bladder Cancer Cells.

Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells.

The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined.

After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which were more resistant to carboplatin, the cell viability was significantly (p<0.05) reduced in the presence of γδ T-cells.

Our findings showed that γδ T-cell therapy has potent benefit in cancer treatment.

Anticancer research. 2020 Sep [Epub]

Yueh Pan, Ya-Hsu Chiu, Shao-Chih Chiu, DER-Yang Cho, Liang-Ming Lee, Yu-Ching Wen, Jacqueline Whang-Peng, Chi-Hao Hsiao, Ping-Hsiao Shih

Department of Urology, Wan Fang Hospital, Taipei, Taiwan, R.O.C., Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C., Cancer Center, Wan Fang Hospital, Taipei, Taiwan, R.O.C., Department of Urology, Wan Fang Hospital, Taipei, Taiwan, R.O.C. ., Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C. .