Bladder cancer (BLCA) is a common malignant cancer, and it is the most common genitourinary cancer in the world. The recurrence rate is the highest of all cancers, and the treatment of BLCA has only slightly improved over the past 30 years. Genetic and environmental factors play an important role in the development and progression of BLCA. However, the mechanism of cancer development remains to be proven. Therefore, the identification of potential oncogenes is urgent for developing new therapeutic directions and designing novel biomarkers for the diagnosis and prognosis of BLCA. Based on this need, we screened overlapping differentially expressed genes (DEG) from the GSE7476, GSE13507, and TCGA BLCA datasets. To identify the central genes from these DEGs, we performed a protein-protein interaction network analysis. To investigate the role of DEGs and the underlying mechanisms in BLCA, we performed Gene Ontology (GO) and Kyoto Gene and Genomic Encyclopedia (KEGG) analysis; we identified the hub genes via different evaluation methods in cytoHubba and then selected the target genes by performing survival analysis. Finally, the relationship between these target genes and tumour immunity was analysed to explore the roles of these genes. In summary, our current studies indicate that both cell division cycle 20 (CDC20) and abnormal spindle microtubule assembly (ASPM) genes are potential prognostic biomarkers for BLCA. It may also be a potential immunotherapeutic target with future clinical significance.
BioMed research international. 2020 Jan 23*** epublish ***
Yingkun Xu, Guangzhen Wu, Jianyi Li, Jiatong Li, Ningke Ruan, Liye Ma, Xiaoyang Han, Yanjun Wei, Liang Li, Hongge Zhang, Yougen Chen, Qinghua Xia
Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China., Department of Urology, The First Affiliated Hospital of Dalian Medical University, Dalian, China., Department of Nephrology, Shandong Provincial Hospital, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, China., The Nursing College of Zhengzhou University, Zhengzhou, China., Department of Liver Transplantation and Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China., Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China., Shangdong Academy of Medical Sciences, Jinan University, Jinan, China., Department of Urology, Tengzhou Hospital of Traditional Chinese Medicine, Tengzhou, China.