Bladder cancer (BCa) is a tumor associated with high morbidity and mortality and its incidence is increasing worldwide. However, the pathogenesis of bladder cancer is not well understood.
To further illustrate the molecular mechanisms involved in the pathogenesis of BCa and identify potential therapeutic targets, we combined the transcriptomic analysis with RNA sequencing and tandem mass tags (TMT)-based proteomic methods to quantitatively screen the differentially expressed genes and proteins between bladder cancer tissues (BC) and adjacent normal tissues (AN).
Transcriptome and proteome studies indicated 7094 differentially expressed genes (DEGs) and 596 differentially expressed proteins (DEPs) between BC and AN, respectively. GO enrichment analyses revealed that cell adhesion, calcium ion transport, and regulation of ATPase activity were highly enriched in BCa. Moreover, several key signaling pathway were identified as of relevance to BCa, in particular the ECM-receptor interaction, cell adhesion molecules (CAMs), and PPAR signaling pathway. Interestingly, 367 genes were shared by DEGs and DEPs, and a significant positive correlation between mRNA and translation profiles was found.
In summary, this joint analysis of transcript and protein profiles provides a comprehensive reference map of gene activity regarding the disease status of BCa.
Genes & genomics. 2019 Oct 01 [Epub ahead of print]
Songbai Liao, Minglin Ou, Liusheng Lai, Hua Lin, Yaoshuang Zou, Yonggang Yu, Xuede Li, Yong Dai, Weiguo Sui
The First School of Clinical Medicine, Southern Medical University, Guangdong, 510515, China., Guangxi Key Laboratory of Metabolic Diseases Research, Affiliated No.924 Military Hospital, Southern Medical University, Guangxi, 541002, China., Department of Urology, Affiliated No.924 Military Hospital, Southern Medical University, Guangxi, 541002, China., Clinical Medical Research Center, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, 518020, Guangdong, People's Republic of China., The First School of Clinical Medicine, Southern Medical University, Guangdong, 510515, China. .