A colorimetric immunosensor based on self-linkable dual-nanozyme for ultrasensitive bladder cancer diagnosis and prognosis monitoring.

We developed self-linkable Prussian blue (PB)-incorporated magnetic graphene oxide (PMGO) as a peroxidase-mimicking nanozyme with high oxidizability to 3,3',5,5'-tetramethylbenzidine (TMB), which generates significant absorption intensity for the colorimetric immunosensing of apolipoprotein A1 (ApoA1) in early bladder cancer (BC) diagnosis and prognosis monitoring. The ultrasensitive immunosensor was constructed using an ApoA1 antibody (AbApoA1)-functionalized chip (biochipApoA1) and self-linkable peroxidase-mimicking, PB-incorporated magnetic graphene oxide (PMGO). After incubating the sample and capturing ApoA1 proteins captured on the biochipApoA1, the PMGO was functionalized with AbApoA1, and then mouse IgG (PMGO-1), rabbit anti-mouse IgG antibody (PMGO-2), and goat anti-rabbit IgG antibody (PMGO-3) were added together. We envisioned that each captured ApoA1 protein would allow the retention of a large amount of PMGO through a self-linking process to amplify the colorimetric signal of TMB in the presence of H2O2. The linear detection range could be obviously widened in the presence of self-linkable PMGO-from 0.05 ng/mL to 100 ng/mL-compared with the group without signal amplification (from 1 ng/mL to 100 ng/mL). Our immunosensor analysis of ApoA1 in the urine of BC patients and healthy individuals was highly correlated with enzyme-linked immunosorbent assay measurements; moreover, the ApoA1 concentrations of patients with high-grade BC were significantly higher than those of patients with low-grade BC. After standard clinical treatment, a significant drop of ApoA1 concentration occurred in urine that was lower than the cut-off concentration, suggesting potential clinical applications of the new self-linkable PMGO-generating colorimetric immunosensor in early BC diagnosis and prognosis monitoring.

Biosensors & bioelectronics. 2018 Nov 16 [Epub ahead of print]

Chen Peng, Mu-Yi Hua, Nan-Si Li, Ying-Pei Hsu, Ying-Tzu Chen, Cheng-Keng Chuang, See-Tong Pang, Hung-Wei Yang

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan., Department of Chemical and Materials Engineering, Biosensor Group, Biomedical Engineering Research Center; and Healthy Aging Research Center, Chang Gung University, Taoyuan 33302, Taiwan; Department of Neurosurgery, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, Taiwan., Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan., Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, Taiwan; School of Medicine, Chang Gung University, Taoyuan 33302, Taiwan., Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Linkou, Taoyuan 33305, Taiwan; School of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. Electronic address: ., Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. Electronic address: .

E-Newsletters

Newsletter subscription

Free Daily and Weekly newsletters offered by content of interest

The fields of GU Oncology and Urology are rapidly advancing. Sign up today for articles, videos, conference highlights and abstracts from peer-review publications by disease and condition delivered to your inbox and read on the go.

Subscribe