miR-145-5p inhibits the proliferation and migration of bladder cancer cells by targeting TAGLN2

MicroRNA-145-5p (miR-145-5p) is found to be involved in tumor development and progression. However, there are few studies on the effects of miR-145-5p on bladder cancer (BC). The role of miR-145-5p in BC was predicted by analysis of cell proliferation and migration in this study. The miR-145-5p and transgelin-2 (TAGLN2) expressions were evaluated via reverse transcription-quantitative PCR (RT-qPCR) or western blot analysis. The MTT and Transwell assay assessed cell proliferation and migration. TAGLN2 targeted to miR-145-5p was determined using luciferase assays. The results showed that the miR-145-5p downregulation was found in BC. miR-145-5p overexpression inhibited cell proliferation and migration in BC. Moreover, miR-145-5p directly targeted TAGLN2, and TAGLN2 expression was increased in BC. In addition, the high expression of TAGLN2 promoted cell proliferation and migration in BC. miR-145-5p appeared to regulate TAGLN2 in BC, and it also inhibited the cell proliferation and migration. The novel miR-145-5p/TAGLN2 axis may provide new therapeutic implications for BC.

Oncology letters. 2018 Sep 12 [Epub]

Haijian Zhang, Meijuan Jiang, Qingjun Liu, Zhixing Han, Yuqian Zhao, Shiqi Ji

Department of Urology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China., Department of Neurology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China.