Lower urinary tract symptoms (LUTS) are highly prevalent among the elderly and negatively impact quality-of-life. Since caffeinated beverages are enjoyed worldwide and the relationship between LUTS and caffeine is still not fully understood, it would be of particular interest to examine the underlying mechanisms that drive caffeine's influence on LUTS development and progression. The aim of this study was to characterize the effects of caffeine on hTert immortalized normal bladder epithelial cells by investigating whether exposure to caffeine can cause potential changes in the bladder proteome and/or biological pathways. Labeled LC-MS/MS proteomic analysis found 57 proteins as being differentially expressed in caffeine-treated bladder epithelial cells, compared to controls; this included 32 upregulated and 25 downregulated proteins. Further functional gene enrichment analysis revealed that caffeine affected major biological pathways, including those for "muscle contraction" and "chromatin assembly". These findings provide new scientific insights that may be useful in future studies investigating the role of caffeine in bladder dysfunctions. This article is protected by copyright. All rights reserved.
Proteomics. 2018 Sep 19 [Epub ahead of print]
Muhammad Shahid, Minhyung Kim, Austin Yeon, Allen M Andres, Sungyong You, Jayoung Kim
Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA., Department of Medicine, Cedars Sinai Medical Center, Los Angeles, CA, USA.