Correlation Of Immune Cells And Cytokines In The Tumor Microenvironment With Elevated Neutrophil-To-Lymphocyte Ratio In Blood: An Analysis Of Muscle-Invasive Bladder Cancer

We investigated the relationship between Neutrophil-to-lymphocyte ratio (NLR) and the quantities of neutrophil elastase, CD3, Foxp3, and CD204 in tumor-infiltrating cells and circulating cytokines (IL-2, -6, -8, 17a, and TGF-β) in muscle-invasive bladder cancer. IL-6 and IL-8 levels showed a significant correlation with NLR in blood and Foxp3+ cells around the tumor. After co-culture of peripheral blood cells with bladder cancer cell lines, the induction of regulatory T cell (Treg) was higher in T24 whose IL-6 and IL-8 levels were higher. High NLR correlates with increased IL-6 and IL-8 and Treg expression.

Cancer investigation. 2018 Sep 10 [Epub ahead of print]

Yosuke Morizawa, Makito Miyake, Keiji Shimada, Shunta Hori, Yoshihiro Tatsumi, Yasushi Nakai, Sayuri Onishi, Nobumichi Tanaka, Noboru Konishi, Kiyohide Fujimoto

a Department of Urology , Nara Medical University , Kashihara , Japan., b Department of Pathology , Nara City Hospital , Nara , Japan., c Department of Pathology , Nara Medical University , Kashihara , Japan.