Uncomplicated urinary tract infections, especially those caused by Escherichia coli, have historically been widely studied. However, complicated urinary tract infections are presenting ever increasing healthcare challenges, particularly with Proteus mirabilis. P. mirabilis is often found on indwelling urinary catheters causing monomicrobial and polymicrobial catheter-associated urinary tract infection (CAUTI). Widespread antibiotic resistance, combined with the ability of P. mirabilis to form urinary calculi during infection, warrants further investigation of this pathogen and its host interaction in an infection model that more closely mimics the presence of an indwelling urinary catheter. Here, we describe the methods necessary to establish a murine model of P. mirabilis CAUTI.
Methods in molecular biology (Clifton, N.J.). 2019 Jan [Epub]
Sara N Smith, Chelsie E Armbruster
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA. .