Regeneration of native-like neo-urinary tissue from non-bladder cell sources - Abstract

Tengion Inc, Bioprocess Research & Assay Development, Winston Salem, North Carolina, United States.


Urinary pathology requiring urinary diversion, partial or full bladder replacement, is a significant clinical problem affecting approximately 14,000 individuals annually in the United States alone. The use of gastrointestinal tissue for urinary diversion or bladder reconstruction/replacement surgeries is frequently associated with complications. To try and alleviate or reduce the frequency of these complications, tissue engineering and regenerative medicine strategies have been developed using bio-absorbable materials seeded with cells derived from the bladder. However, bladder-sourced cells may not always be suitable for such applications, especially in patients with bladder cancer. In this study, we describe the isolation and characterization of smooth muscle cells from porcine adipose and peripheral blood that are phenotypically and functionally indistinguishable from bladder-derived smooth muscle cells. In a pre-clinical GLP study, we demonstrate that autologous adipose- and peripheral blood-derived smooth muscle cells may be used to seed synthetic, biodegradable tubular scaffold structures and that implantation of these seeded scaffolds into a porcine cystectomy model leads to successful de novo regeneration of a tubular neo-organ composed of urinary-like neo-tissue which is histologically identical to native bladder. The ability to create urologic structures de novo from scaffolds seeded by autologous adipose- or peripheral blood-derived smooth muscle cells will greatly facilitate the translation of urologic tissue engineering technologies into clinical practice.

Written by:
Basu J, Jayo MJ, Ilagan R, Guthrie KI, Sangha N, Genheimer C, Quinlan S, Payne R, Knight T, Rivera EA, Jain D, Bertram T, Ludlow JW.   Are you the author?

Reference: Tissue Eng Part A. 2011 Dec 2. Epub ahead of print.
doi: 10.1089/ten.TEA.2011.0569

PubMed Abstract
PMID: 22136657 Urologic Trauma & Reconstruction Section