The role of intrinsic pathway in apoptosis activation and progression in Peyronie's disease - Abstract

Peyronie's disease (PD) is characterized with formation of fibrous plaques which result in penile deformity, pain, and erectile dysfunction.

The aim of this study was to investigate the activation of the intrinsic apoptotic pathway in plaques from PD patients. Tunica albuginea from either PD or control patients was assessed for the expression of bax, bcl-2 and caspases 9 and 3 using immunohistochemistry and by measurement of apoptotic cells using TUNEL assay. Bax overexpression was observed in metaplastic bone tissue, in fibroblasts, and in myofibroblast of plaques from PD patients. Little or no bcl-2 immunostaining was detected in samples from either patients or controls. Caspase 3 immunostaining was very strong in fibrous tissue, in metaplasic bone osteocytes, and in primary ossification center osteoblasts. Moderate caspase 9 immunostaining was seen in fibrous cells plaques and in osteocytes and osteoblasts of primary ossification centers from PD patients. Control samples were negative for caspase 9 immunostaining. In PD patients the TUNEL immunoassay showed intense immunostaining of fibroblasts and myofibroblasts, the absence of apoptotic cells in metaplasic bone tissue and on the border between fibrous and metaplastic bone tissue. Apoptosis occurs in stabilized PD plaques and is partly induced by the intrinsic pathway.

Written by:
Loreto C, La Rocca G, Anzalone R, Caltabiano R, Vespasiani G, Castorina S, Ralph DJ, Cellek S, Musumeci G, Giunta S, Djinovic R, Basic D, Sansalone S.   Are you the author?
Department of Bio-Medical Sciences, Anatomy and Histology Section, University of Catania, Via S. Sofia 87, 95123 Catania, Italy; Department of Experimental Biomedicine and Clinical Neuroscience, University of Palermo, 90100 Palermo, Italy; Department G.F. Ingrassia Section of Anatomic Pathology University of Catania, 95123 Catania, Italy; Department of Urology, School of Medicine Tor Vergata University of Rome, 00133 Rome, Italy; University College London Hospital, London WC1E 6BT, UK; Cranfield Health Cranfield University, MK43 0AL, UK; Sava Perovic Foundation, Center for Genito-Urinary Reconstructive Surgery, 11000 Belgrade, Serbia; Clinic of Urology, Clinical Center Nis, 18000 Nis, Serbia.

Reference: Biomed Res Int. 2014;2014:616149.
doi: 10.1155/2014/616149


PubMed Abstract
PMID: 25197653

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