Benign prostatic hyperplasia (BPH) is an age-related disease, occurring in >70% of men of age >60. Because telomeres and telomerase play a key role in aging and age-related diseases, and certain telomerase gene single nucleotide polymorphisms (SNPs) are shown to be associated with the susceptibility to age-related diseases, we wanted to determine the relationship between BPH and leukocyte telomere length (LTL) and telomere length-related single nucleotide polymorphisms (SNPs) of the telomerase holoenzyme genes.
Peripheral blood was collected from both BPH patients and age-matched healthy male controls and genomic DNA was extracted. rs2736100 and rs2736098 at the TERT and rs12696304 at the TERC locus were analysed using pre-designed TaqMan SNP genotyping assay kits. LTL was determined using qPCR.
Patients with BPH had significantly shorter LTL (1.231 ± 0.532 vs 0.899 ± 0.322, P < 0.001). The genotyping results show similar frequencies in rs2736100, rs2736098 and rs12696304 between healthy and BPH individuals.
Shorter telomeres but not telomerase SNPs at the TERT and TERC loci, are associated with BPH. Short telomeres may promote senescence of a fraction of prostatic epithelial cells, while senescent cells in turn facilitate epithelial and stromal cell proliferation by the senescence-associated secretory phenotype mechanism, thereby eventually leading to BPH development.
International journal of clinical and experimental pathology. 2020 Aug 01*** epublish ***
Guanghui Cheng, Mingkai Dai, Qian Xin, Lina Wang, Feng Kong, Dawei Xu
Central Research Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University Jinan 250033, PR China., Engineering Laboratory of Urinary Organ and Functional Reconstruction of Shandong Province 250013, PR China., Department of Medicine, Division of Hematology, Center for Molecular Medicine and Bioclinicum, Karolinsk Institutet and Karolinska University Hospital Solna Stockholm, SE-17176, Sweden.