Intraurethral injection with LPS: an effective experimental model of prostatic inflammation

Chronic inflammation has been recognized as having a prominent role pathogenesis of benign prostatic hyperplasia (BPH) and cancer. It is believed that chronic inflammation induces prostatic fibromuscular growth. This correlation has been clearly illustrated by both in vivo and in vitro studies; however, current experimental models of BPH require complex surgery or hormonal treatment. Therefore, the aim of the present study was to propose a new murine model of BPH/prostatitis induced by intraurethral injection of LPS.

Male Swiss and C57Bl/6 mice were then sacrificed 3, 7, 10, and 14 days after intraurethral injection of LPS. The prostates were quickly dissected and fixed for morphological and immunohistochemical analyses.

The results showed that LPS played an important role in the cell proliferation of the prostate. Histological and ultrastructural analysis showed epithelial hyperplasia, clear stromal cells, little inflammatory infiltration, and heavy bleeding. Treatment with LPS also promoted the increase of growth factor (FGF-7 and TGF-β), α-actin, and proinflammatory cytokines (IL-1, IL-6, IL-17), both in the stroma and epithelium.

According to the present findings, it can be concluded that the intraurethral administration of LPS promotes tissue remodeling, as well as stimulating the pattern of pro-inflammatory cytokines, and therefore, constitutes an effective experimental model of BPH/inflammation.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 2017 Nov 18 [Epub ahead of print]

Fabiana Oliveira Dos Santos Gomes, Amanda Costa Oliveira, Edlene Lima Ribeiro, Bruna Santos da Silva, Laise Aline Martins Dos Santos, Ingrid Tavares de Lima, Amanda Karolina Soares E Silva, Shyrlene Meiry da Rocha Araújo, Terezinha Gonçalves, Mario Ribeiro de Melo-Junior, Christina Alves Peixoto

Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil., Pós-graduação em Ciências Biológicas da Universidade Federal de Pernambuco (UFPE), Recife, Brazil., Ultrastructure Laboratory, Instituto Aggeu Magalhães, Centro de Pesquisas Aggeu Magalhães (CPqAM-FIOCRUZ), Fundação Oswaldo Cruz, Av. Moraes Rego s/n, Cidade Universitária, Recife, PE, 50670-420, Brazil. .