Quantitative Analysis of the Seminal Plasma Proteome in Secondary Hypogonadism.

In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.

Journal of clinical medicine. 2019 Dec 03*** epublish ***

Giuseppe Grande, Federica Vincenzoni, Francesca Mancini, Ferran Barrachina, Antonella Giampietro, Massimo Castagnola, Andrea Urbani, Rafael Oliva, Domenico Milardi, Alfredo Pontecorvi

International Scientific Institute "Paul VI", 100168 Rome, Italy., Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, 100168 Rome, Italy., Molecular Biology of Reproduction and Development Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Barcelona, 08036 Barcelona, Spain., Divisione di Endocrinologia, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, 00168 Rome, Italy., Laboratorio di Proteomica e Metabolomica, IRCCS Fondazione Santa Lucia, 100168 Rome, Italy.