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WCE 2017: Role of Chaperone Proteins in Obstructed Renal Pelvis

Vancouver, Canada (UroToday.com) Dr. Horacio Sanguinetti and colleagues evaluated the expression of Heat Shock Proteins (HSP), or Chaperone proteins, in patients with an obstructed renal pelvic. In previous studies, an increase of HSP were examined in obstructed lab rat and human bladder. HSP expression is important for injured cells to prevent themselves from folding and denaturalizing. It is considered as a protective mechanism at the cellular level. 

The study focused on the expression of HSP 27 and 70 in renal pelvis. There were three sample groups. Group 1 (n = 20) was samples of renal pelvis of patients with pyeloplasty for ureteropelvic junction (UPJ) obstruction. Group 2 (n = 14) was samples of renal pelvis from nephrectomies for hydronephrosis. Group 3 (n = 16) was samples of non-obstructed renal pelvis from nephrectomies for kidney cancer. Immunolabeling was used to evaluate the expression of HSP 27 and 70.

Data analysis showed that the percentage of HSP 27 positive strain in UPJ obstruction samples (18.9 ± 2.7) was significantly higher in hydronephrosis samples (5.5 ± 1.8) and in non-obstructed samples (11.4 ± 2.7), and the percentage in hydronephrosis samples was significantly lower than in non-obstructed samples. The percentage of HSP 70 positive strain in UPJ obstruction samples (16.2 ± 2.1) was significantly higher in hydronephrosis samples (2.5 ± 1.0) and in non-obstructed samples (9.1 ± 1.4), and the percentage in hydronephrosis samples was significantly lower than in non-obstructed samples.    

Dr. Sanguinetti concluded that there was an increase of the HSP 27 and 70 in the obstructed renal pelvis of the UPJ obstruction and a decrease of the HSP 27 and 70 in the renal pelvis in kidneys with hydronephrosis. He suggested that the values of HSP could serve as a useful marker for the prognostic of kidney’s obstruction. 

Presented by: Horacio Sanguinetti  

Authors: Horacio Sanguinetti, Jorge Toblli; Norberto Osvaldo Bernardo, Osvaldo Mazza
Affiliation: Hospital de Clinicas José de San Martin

Written by: Long Hoang, Department of Urology, University of California-Irvine, at 35th World Congress of Endourology– September 12-16, 2017, Vancouver, Canada.